Purchase of LC-MS/MS mass spectrometer and UHPLC
Freiwillige Ex-ante-Transparenzbekanntmachung
Lieferauftrag
Abschnitt I: Öffentlicher Auftraggeber/Auftraggeber
Nationale Identifikationsnummer: DE21H
Postanschrift:[gelöscht]
Ort: Neuherberg
NUTS-Code: DE21H München, Landkreis
Postleitzahl: 85764
Land: Deutschland
Kontaktstelle(n):[gelöscht]
E-Mail: [gelöscht]
Telefon: [gelöscht]
Internet-Adresse(n):
Hauptadresse: www.helmholtz-muenchen.de
Abschnitt II: Gegenstand
Purchase of LC-MS/MS mass spectrometer and UHPLC
The system must:
-perform high-throughput targeted quantitative lipidomics, via dynamic MRM/SRM based on retention time, with analyte-specific time window, option to weight the dwell time for specific analytes, quantification of hundreds of mass transitions simultaneously, in both negative and positive mode in a single analytical run.
-Ion source must accept either an electrospray ionization (ESI) probe or atmospheric pressure chemical ionization (APCI) probe, including a heated ESI probe for use in the ion source @flow rates from 100 to 500 µl/min without splitting at broad flow range from 1 μL/min to up to 3 mL/min with interchangeable probes and electrodes.
-have a linear accelerator trap and triple quadrupole functionality; prove to be highly efficient at collecting and retaining ions from the ESI spray plume, while removing the gas molecules and neutral ions, ensuring a superior enhanced ion generation/ultra-high sensitivity.
-Central control for the LC AD system/AC column oven
The system must:
-perform full scan MS/selected ion monitoring for both Q1 and Q3,product ion scan,precursor ion scan,neutral loss or gain scan,MRM,scheduled MRM,enhanced MS scan,enhanced product ion scan,enhanced resolution (EPI) scan,MS3 scan,MRM3 scan and trap scan modes
-deliver ultra-high sensitivity,in EPI mode ≤10 fg on column with S/N > 200.Proven sensitivity in MRM3 mode ≤10 fg on column with S/N > 30, in MRM mode (positive and negative ESI,in the same injection),allowing low-level trace analytes (between 8 fg and 1 pg on column,S/N > 1,500.000) to be monitored
-have a software that provides automatic,dynamic adjustment of the acquiring MRM transitions
-capture a multitude of information (MRM and sMRM) within a single analysis @linear dynamic range with a six orders of magnitude coverage
-detect broad mass range from 5 to 2000 Da in triple quadrupole mode,offer a mass range of 50-2000 Da in linear ion trap mode; mass stability of 0.1 Da over 24 hours
-Triple quad scan speed 12,000 Da/sec; linear ion trap scan speed of 20,000 Da/sec.Polarity continuously switching 5 msec,in MRM and sMRM modes,minimum MRM dwell time 1 msec. MRM acquisition of 500 MRM/sec
-scan 100 MRM transitions followed by a precursor scan (500 Da) and a neutral loss scan (500 Da) followed by two EPI scans (500 Da) in less than 1.5 seconds,analyzing large batches of lipid extracts over long periods (≥ 1100 hours),without performance degradation,of rapidly switching between positive and negative ion detection,ideally ≤5 msec in MRM and scheduled MRM modes,of detecting a broad spectrum of lipid species (~2000),including PI/LPI,PC/LPC,PE/LPE,PS/LPS,PG/LPG,MAG,DAG,TAG,CL,CE,CER,DCER,HCER,LHCER and SM,in a variety of matrices,in positive and negative ion mode,of high-throughput,targeted,quantitative lipid analysis with low femtogram sensitivity,with LLOQ between 8 fg and 1 pg on column and %CVs smaller than 20%,of monitoring a broad spectrum of lipid species (≥110),including endocannabinoids
-Two pumps,each for a specific solvent and a pressure rating of 1300 bar.Micro volume double plunger pump (10 µL/stroke).Constant flow of 0.0001 to 3 mL/min (10-1300 bar),3.0001 to 5 mL/min (10-800 bar),and 5.0001 to 10 mL/min (10-220 bar).Flow rate accuracy ±1% (1 mL/min,800 bar).Flow rate precision RSD < 0.06% or 0.02 min.Mix ratio setting range 0-100% (in 0.1% steps). Concentration accuracy ±0.5% (at 0.5-3 mL/min).Flow rates 0.0001 to 10 mL/min.Two solvent selections (per pump) with solvent selection valve. 10 µm suction filter. 5 µm mesh line filter,capacity 70 µL
-UPLC direct cooled autosampler rated to 1300 bar (18850 psi).Temp-control from 4°C to 35°C.Temp- accuracy ±3°C.Variable injection volume flow through design with no sample loss during injection. Multiple rinse solvents (internal&external needle) that can provide a typical carryover value of < 0.002%.Sample capacity of 105 x 1.5 mL vials. Injection volume 0.1 to 50 µL (0.1 to 0.9 µL in 0.1 µL increments,1 to 50 µL in 1 µL increments).Injection volume precision RSD ≤ 0.25%.; accuracy ±1%.Sample aspiration rate 0.1 to 15 µL /sec (0.1 µL /sec increments).Rinse aspiration 1 to 35 µL/sec,(0.1 µL /sec increments).Four rinse solution types (to 2 for needle surface,3 for inner surface).Nominal injection cycle < 14 seconds
-Forced air circulation 6 columns oven with cooling.Temp. from 10°C below ambient to 85°C.Temp. precision ± 0.1°C.Temp. from 4°C to 35°C and humidity from 20% to 85%
-The differential mobility separation (DMS) device must: -consist of an atmospheric pressure mobility cell,-acquire data for a single MRM transition in 25 msec including the inter-scan pause time of 20 msec,-have an integrated liquid pump for the purpose of introducing chemical modifiers into the mobility cell,-be user mountable and dis-mountable w/o the use of tools or breaking vacuum,-dis-/mountable in less than 5 minutes,-separate different lipid species and to improve lipid quantification
-have dedicated PC Hard- and Software
The system must prove MS3 capabilities: quantification in MRM3 for enhanced compound selectivity, eliminating chromatographic interference, resulting in superior analytical performance, enable fast separations without long LC run times to eliminate/reduce background interference. Combination of MRM/SRM modes and fragment spectra recording for simultaneous quantification/identification.
Abschnitt IV: Verfahren
- Die Bauleistungen/Lieferungen/Dienstleistungen können aus folgenden Gründen nur von einem bestimmten Wirtschaftsteilnehmer ausgeführt werden:
- nicht vorhandener Wettbewerb aus technischen Gründen
The combination of DMS by SelexION and MS3 capabilities of the QTrap instrumentation enable an unique in-depth profiling and unique quantitation of lipid species with required sensitivity and broader lipid coverage.
Due to the compatibility of the existing systems and the planned purchase of Sciex QTrap 6500+ with SelexION, the instruments can serve as back up for each in case of failure and allow to go on with time-critical measurements.
All instruments have to be combined with the same Sciex Exion AD LC system, allowing seamless method transfer between all the instruments without readjustment of chromatographic separation.
Abschnitt V: Auftragsvergabe/Konzessionsvergabe
Ort: Darmstadt
NUTS-Code: DE711 Darmstadt, Kreisfreie Stadt
Land: Deutschland
Abschnitt VI: Weitere Angaben
Ort: Bonn
Postleitzahl: 53123
Land: Deutschland
E-Mail: [gelöscht]
Telefon: [gelöscht]
Fax: [gelöscht]
VI.4.3) Application of legal remedies Detailed procedures on deadlines for the application of legal remedies:
According to § 160 (3) Clause 1 German Act against Restraints of Competition (GWB) an application for review is inadmissible if 1. the applicant became aware of the claimed violation of public procurement provisions before filing the application for review, but did not complain to the contracting authority within a time limit of 10 calendar days; the expiry of the time limit under § 134(2) remains unaffected, 2. violations of public procurement provisions which become apparent from the tender notice are not notified to the contracting authority by the end of the time limit for the application or the submission of a tender specified in the notice 3. violations of public procurement provisions which only become apparent from the procurement documents are not notified to the contracting authority by the end of the time limit for the application or the submission of a tender specified in the notice, 4. more than 15 calendar days have expired since receipt of notification from the contracting authority that it is unwilling to redress the objection. Sentence 1 shall not apply to an application under § 135(1) no. 2 to have the contract declared ineffective. § 134(1) sentence 2 shall remain unaffected. According to §134 (1) German Act against Restraints of Competition (GWB) public contracting authorities shall inform the unsuccessful tenderers in writing and without delay of the name of the successful undertaking, the reasons for the rejection of their tenders and of the earliest date of the conclusion of the contract. This shall also apply to candidates who were not informed of the rejection of their tenders before notification of the decision on the award was sent to the successful tenderers. According to § 134 (2) German Act against Restraints of Competition (GWB) a contract may be concluded at the earliest 10 calendar days after the information pursuant to paragraph 1 has been sent (by fax, e-mail, electronically or via the e-tendering online portal. The standstill period shall begin on the day after which the contracting authority dispatches the information; the date of receipt by the tenderer and candidate in question shall be irrelevant.