Delivery, installation and training incl. warranty of Mass spectrometer, DMS Device, UHPLC-System
Bekanntmachung vergebener Aufträge
Ergebnisse des Vergabeverfahrens
Lieferauftrag
Abschnitt I: Öffentlicher Auftraggeber
Nationale Identifikationsnummer: DE21H
Postanschrift: Ingolstädter Landstraße 1
Ort: Neuherberg
NUTS-Code: DE21H München, Landkreis
Postleitzahl: 85764
Land: Deutschland
E-Mail:
Telefon: +49 89318743216
Internet-Adresse(n):
Hauptadresse: www.helmholtz-muenchen.de
Abschnitt II: Gegenstand
Delivery, installation and training incl. warranty of Mass spectrometer, DMS Device, UHPLC-System
Delivery, installation and training incl. warranty of Mass spectrometer, DMS Device, UHPLC-System.
Mass spectrometer, DMS Device and UHPLC-System
.Specifications mass spectrometer (MS/MS)
- The ion source must accept either an electrospray ionization (ESI) probe or an atmospheric pressure chemical ionization (APCI) probe. The source must be compatible with a broad flow range from microflow (1 μL/min) to analytical flow (up to 3 mL/min) with interchangeable probes and electrodes. The instrument must be capable of enabling fast interchanges between high and low flow rates.
- The LC-MS/MS instrument must be compatible with and controlled by a software that provides automatic, dynamic adjustment of the acquiring MRM transitions such that only compounds that elute within a specified time window are monitored. In addition, the process of creating the acquisition method to utilize this feature must accept a simple list of compound names, MRM transitions, and expected retention times, and use this list to enable dynamically schedule MRM scans during the experiment. The instrument must be capable to perform high-throughput, targeted quantitative lipidomics via dynamic MRM/SRM, based on retention time, with analyte-specific time windows, with the option to weight the dwell time for specific analytes, allowing the quantification of hundreds of mass transitions simultaneously, in both negative and positive mode in a single analytical run.
- The instrument must provide MS3 capabilities, allowing quantification in MRM3 for enhanced compound selectivity, eliminating chromatographic interference and resulting in superior analytical performance compared to traditional MRM. The device must enable fast separations without the need for long LC run times to eliminate/reduce background interference. Moreover, combination of MRM/SRM modes and fragment spectra recording for simultaneous quantification and identification is required.
- The instrument must be capable of performing full scan MS and selected ion monitoring for both Q1 and Q3, product ion scan, precursor ion scan, neutral loss or gain scan, MRM, scheduled MRM (sMRM), enhanced MS scan, enhanced product ion scan, enhanced resolution (EPI) scan, MS3 scan, MRM3 scan, and trap scan modes.
Specifications DMS Device
- The Differential Mobility Spectrometry (DMS) device must consist of an atmospheric pressure mobility cell located prior to the single thin aperture from atmosphere into the vacuum chamber. The DMS device must be able to acquire data for a single MRM transition in 25 msec including the inter-scan pause time of 20 msec.
Specifications UHPLC-System
- The LC system must provide exceptional chromatographic resolution with virtually undetectable carryover.
- Controller module. Central communication and control module for the LC AD system and AC column oven. Modules should connect via fiber optic cables for easy connectivity.
- Degasser. Five channel solvent degasser with inline membrane degasser utilizing Teflon AF for degassing of HPLC mobile phases. Volume of 400 μL per line.
General:
- A minimum of 2 years full warranty after installation including all spare parts and service calls and one preventive maintenance per year.
- Possibility for subsequent full service contract after expiration of the warranty, which includes all spare parts and service calls and one preventive maintenance per year.
QTOF-MS Mass Spectrometer & UHPLC-System
Specifications QTOF-MS
- The QTOF-MS must be suitable for both targeted and non-targeted metabolomic analyses.
- The QTOF-MS must have the capability to use different ion sources for different flow rates of the flow agents. A flow rate range of 5 µL/min to 3 mL/min must be covered. Optionally, alternative ion sources for lower flow rates shall be available.
- The QTOF-MS must have temperature stabilization of the flight tube by means of thermostatization.
- The QTOF-MS must have an isolating quadrupole that can isolate ions with a mass-to-charge ratio in the range of m/z 5 - 2250.
- The QTOF-MS must allow maximum scan speeds of >= 25 mass spectra per second in MS1 and of >100 mass spectra per second in MS2.
- For acquisition of MS2 data, the ion yield in the TOF (duty cycle) must be >=90%.
- The mass resolution of the QTOF-MS must be > 40,000.
- The QTOF-MS must allow multiple fragmentation modes, for example to distinguish between positional isomers (e.g. sn-1/sn-2 isomers in glycerophospholipids or different phosphorylation positions). It must allow for CID (Collision-Induced Dissociation) and EAD (Electron Activated Dissociation) fragmentation techniques, or equivalent, for fragmentation of small, singly charged analytes. For EAD or equivalent technology, the collision energy must be freely selectable between 0 and 25eV.
- Fragmentation data (MS2 data) must be generatable in both data-dependent and data-independent modes.
In a data-independent fragmentation mode, it must be possible to divide the entire mass range into up to a maximum of 200 individual windows per scan cycle in order to increase data quality. The mass ranges of the windows are to be selectable thereby both in constant and flexible width.
- It muss be possible to perform an integrated, automated calibration of MS1 and MS2 during data acquisition or after method changes to ensure maximum stability of mass accuracy.
- The QTOF-MS must have a linear dynamic range in positive and negative ion modes of >=5 orders of magnitude, with dynamic range within a scan of up to 4 orders of magnitude.
- The mass accuracy of the QTOF-MS must be within 2 ppm relative mean deviation over a 12 hour measurement period.
When calibrated internally, the QTOF-MS must have a mass accuracy less than 0.5 ppm for all peaks in a calibrated mass range of m/z 100-2000.
Specifications UHPLC-System
- The UHPLC instrument used as the front-end of the UHPLC-QTOF-MS system must consist of the following components: A binary high-pressure pump with degassing unit, an automatic sampler, a column thermostat and a tray to store the eluent bottles, and a solvent selection valve.
- For the pump, the pressure range must be ≥ 1300 bar. It must be possible to mix two eluent channels by means of high-pressure gradient mixing. Several eluents shall be available for selection per channel via a solvent valve. An automatic degassing unit must be integrated in the system.
The automatic sampler must be able to inject volumes from 0 to 40 µL without modification. Samples should be stored refrigerated at 4°C. This must be guaranteed for different sample vessel formats, such as different LC vials or 96- and/or 384-well plates.
- The entire system must be designed as a biocompatible system.
General:
- A minimum of 2 years full warranty after installation including all spare parts and service calls and one preventive maintenance per year.
- Possibility for subsequent full service contract after expiration of the warranty, which includes all spare parts and service calls and one preventive maintenance per year.
Abschnitt IV: Verfahren
- Die betreffenden Erzeugnisse werden gemäß den in der Richtlinie genannten Bedingungen ausschließlich für Forschungs-, Versuchs-, Untersuchungs- oder Entwicklungszwecke hergestellt
The only systems on the market which can comply with the described specifications as written for lot 1 and lot 2 have been contracted as a result of a comprehensive market investigation.
Abschnitt V: Auftragsvergabe
Delivery, installation and training incl. warranty of Mass spectrometer, DMS Device, UHPLC-System
Ort: Darmstadt
NUTS-Code: DE71 Darmstadt
Land: Deutschland
Abschnitt VI: Weitere Angaben
Ort: Bonn
Postleitzahl: 53123
Land: Deutschland
E-Mail:
Telefon: +49 22894990
Fax: +49 2289499163
VI.4.3) Application of legal remedies Detailed procedures on deadlines for the application of legal remedies: According to § 160 (3) Clause 1 German Act against Restraints of Competition (GWB) an application for review is inadmissible if 1. the applicant became aware of the claimed violation of public procurement provisions before filing the application for review, but did not complain to the contracting authority within a time limit of 10 calendar days; the expiry of the time limit under § 134(2) remains unaffected, 2. violations of public procurement provisions which become apparent from the tender notice are not notified to the contracting authority by the end of the time limit for the application or the submission of a tender specified in the notice 3. violations of public procurement provisions which only become apparent from the procurement documents are not notified to the contracting authority by the end of the time limit for the application or the submission of a tender specified in the notice, 4. more than 15 calendar days have expired since receipt of notification from the contracting authority that it is unwilling to redress the objection. Sentence 1 shall not apply to an application under § 135(1) no. 2 to have the contract declared ineffective. § 134(1) sentence 2 shall remain unaffected. According to § 134 (1) German Act against Restraints of Competition (GWB) public contracting authorities shall inform the unsuccessful tenderers in writing and without delay of the name of the successful undertaking, the reasons for the rejection of their tenders and of the earliest date of the conclusion of the contract. This shall also apply to candidates who were not informed of the rejection of their tenders before notification of the decision on the award was sent to the successful tenderers. According to § 134 (2) German Act against Restraints of Competition (GWB) a contract may be concluded at the earliest 10 calendar days after the information pursuant to paragraph 1 has been sent (by fax, e-mail, electronically or via the e-tendering online portal. The standstill period shall begin on the day after which the contracting authority dispatches the information; the date of receipt by the tenderer and candidate in question shall be irrelevant.